Application
IHC-P analysis of human kidney tissue using PPAT antibody at 4.0-8.0μg/ml.Application
WB analysis of HepG2 cells using PPAT antibody at 0.25μg/ml. Lane A : marker Lane B : HepG2 cellsApplication
WB analysis of human skin fibroblasts, HepG2, HEK273 cells, HeLa cells, and hamster CHO K1 cells using PPAT antibody at 1:5000. Lane : 1 :Human skin fibroblast (100μg) 2 : HepG2 (20μg) 3 : HEK293 (20μg) 4 : HeLa (20μg) 5 : Hamster CHO K1 (20μg)| Product Name | PPAT antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPATThe protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region. [provided by RefSeq, Mar 2011] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human PPAT |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanHamster |
| Predicted Reactivity | Cow: 86%; Dog: 86%; Guinea Pig: 86%; Horse: 86%; Human: 100%; Rabbit: 93%; Rat: 79% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATASE PRAT GPAT PPAT |
|---|---|
| Target | PPAT |
| Molecular Weight(MW) | 57 KDa (note) |
Application
IHC-P analysis of human kidney tissue using PPAT antibody at 4.0-8.0μg/ml.
Application
WB analysis of HepG2 cells using PPAT antibody at 0.25μg/ml. Lane A : marker Lane B : HepG2 cells
Application
WB analysis of human skin fibroblasts, HepG2, HEK273 cells, HeLa cells, and hamster CHO K1 cells using PPAT antibody at 1:5000. Lane : 1 :Human skin fibroblast (100μg) 2 : HepG2 (20μg) 3 : HEK293 (20μg) 4 : HeLa (20μg) 5 : Hamster CHO K1 (20μg)| Positive Control | Hamster CHO K1 , HEK273 , HeLa , HepG2 , Human skin fibroblasts |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
