Application
Western blot analysis of mouse liver cytosol extract using at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.| Product Name | PPM1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [p6c7] to PPM1APPM1A is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2C, the prototypical member of the PPM family. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | p6c7 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/250 - 1/1000. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | EC 3.1.3.16 MGC9201 Mpp alpha Protein phosphatase 1A Protein phosphatase 1A magnesium dependent alpha |
|---|---|
| Target | PPM1A |
| Cellular Localization | Cytoplasmic |
| Light Chain Type | kappa |
Application
Western blot analysis of mouse liver cytosol extract using at a dilution of 1/250. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.| Application Notes | ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/250 - 1/1000. Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
