Application
Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (Ab-136).Application
Western blot analysis of lysed extracts from HeLa and 3T3 cells using α-Synuclein (Ab-136).| Product Name | a Synuclein Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NACP, alphaSYN, NACP, SYN, SYUA, alpha-synuclein |
| Product description | May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.1) Takahashi T, et al. J Biol Chem 2003 Oct 24; 278 (43): 42225-33Ahn BH, et al. J Biol Chem 2002 Apr 05; 277 (14): 12334-42Negro A, et al. FASEB J 2002 Feb; 16 (2): 210-2Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119. |
| Immunogen | a-Synuclein (Ab-136) antibody was raised against a peptide sequence around aa. 134~138 (Q-D-Y-E-P) derived from Mouse α-Synuclein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Snca |
|---|---|
| Alternative Names | NACP alphaSYN NACP SYN SYUA alpha-synuclein |
| Molecular Weight(MW) | 18 kDa |
| Tissue Specificity | This antibody detects endogenous level of total α-Synuclein protein. |
| Entrez Gene | 20617 |
|---|---|
| Protein Accession | NP_001035916.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using α-Synuclein (Ab-136).
Application
Western blot analysis of lysed extracts from HeLa and 3T3 cells using α-Synuclein (Ab-136).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
