Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
AARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | AARS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alanine--tRNA ligase, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133}, 6117 {ECO:0000255|HAMAP-Rule:MF_03133}, Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133}, AlaRS {ECO:0000255|HAMAP-Rule:MF_03133}, AARS2 {ECO:0000255|HAMAP-Rule:MF_03133} |
| Product description | Alanyl-tRNA synthetase 2 (AARS2) is an enzyme that catalyzes the chemical reaction: ATP + L-alanine + tRNAAla AMP + diphosphate + L-alanyl-tRNAAla. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis.1) Bonnefond,L.,Biochemistry 44 (12), 4805-4816 (2005) |
| Immunogen | This AARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the Central region of human AARS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AARS2 |
|---|---|
| Alternative Names | Alanine--tRNA ligase mitochondrial {ECO:0000255|HAMAP-Rule:MF_03133} 6117 {ECO:0000255|HAMAP-Rule:MF_03133} Alanyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_03133} AlaRS {ECO:0000255|HAMAP-Rule:MF_03133} AARS2 {ECO:0000255|HAMAP-Rule:MF_03133} |
| Molecular Weight(MW) | 107 kDa |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
AARS2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
