Application
Antibody used in IHC on Human Capan1 at 1:300.Application
Antibody used in WB on Human capan1, HPAF at: 1:1000 (Lane 1. 45ug capan1 cell lysate Lane 2. 45 ug HPAF cell lysate).Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.Application
Antibody used in WB on HePG2 at: 1:1000.Application
Antibody used in WB on Mouse liver at: 1:500 (Lane 1. 60 ug mouse liver extract, Lane 2. 60 ug mouse liver extract, Lane 3. 60 ug mouse liver extract).Application
Antibody used in WB on Human kidney at 0.2-1 ug/ml.| Product Name | ACO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACO1, IRP1, ACONS, HEL60, IREB1, IREBP, IREBP1 |
| Product description | ACO1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.Aconitase 1, also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. Thus, IREB1 plays a central role in cellular iron homeostasis. It was also shown to have aconitase activity, and hence grouped with the aconitase family of enzymes.1) Clarke, S.L., (2006) EMBO J. 25 (3), 544-553. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACO1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ACO1 antibody can be used for detection of ACO1 by ELISA at 1:62500. ACO1 antibody can be used for detection of ACO1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACO1 |
|---|---|
| Alternative Names | ACO1 IRP1 ACONS HEL60 IREB1 IREBP IREBP1 |
| Molecular Weight(MW) | 98 kDa |
Application
Antibody used in IHC on Human Capan1 at 1:300.
Application
Antibody used in WB on Human capan1, HPAF at: 1:1000 (Lane 1. 45ug capan1 cell lysate Lane 2. 45 ug HPAF cell lysate).
Application
Antibody used in IHC on Human Skeletal muscle at 4.0-8.0 ug/ml.
Application
Antibody used in WB on HePG2 at: 1:1000.
Application
Antibody used in WB on Mouse liver at: 1:500 (Lane 1. 60 ug mouse liver extract, Lane 2. 60 ug mouse liver extract, Lane 3. 60 ug mouse liver extract).
Application
Antibody used in WB on Human kidney at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10408 - Fetal Kidney Tissue Lysate |
|---|---|
| Application Notes | ACO1 antibody can be used for detection of ACO1 by ELISA at 1:62500. ACO1 antibody can be used for detection of ACO1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ACO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
