ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-ACO1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA |
| ELISA:1:1000-10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACO1 |
|---|---|
| Gene Synonyms | Aco-1 Acon1 ACONS AH HEL60 IREB1 IREBP IREBP1 IRP1 |
| Gene Full Name | aconitase 1 |
| Gene Summary | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
| Alternative Names | aconitase 1 soluble aconitate hydratase cytoplasmic citrate hydro-lyase cytoplasmic aconitase cytosplasmic aconitase epididymis luminal protein 60 epididymis secretory sperm binding protein ferritin repressor protein IRE-BP 1 iron regulatory protein 1 iron-responsive element-binding protein 1 OTTHUMP00000021176 OTTHUMP00000021177 OTTHUMP00000045233 RNA-binding protein soluble aconitase |
| Molecular Weight(MW) | 98kDa |
| Source | Rabbit |
| Cellular Localization | Cytoplasm, cytosol |
| SwissProt ID | P21399 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.| Application Notes | ELISA:1:1000-10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
