WB
Various lysates were subjected to SDS PAGE followed by western blot with Aconitase 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.WB
Various lysates were subjected to SDS PAGE followed by western blot with Aconitase 1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using Aconitase 1 antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Aconitase 1 antibody at dilution of 1:200 and Omnimabs®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-Aconitase 1 (IP: 4ug; Detection: 1:5000) with HepG2 cells lysate 1925 ug.| Product Name | Aconitase 1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human ACO1 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCIPWB |
| WB:1:1000-1:4000 IHC:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACO1 |
|---|---|
| Gene Synonyms | IRP1 ACONS HEL60 IREB1 IREBP IREBP1 |
| Gene Full Name | aconitase 1 |
| Gene Summary | The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014] |
| Alternative Names | ACO1 ACO 1 EC:4.2.1.3 Citrate hydro-lyase Aconitase1 |
| Molecular Weight(MW) | 98kDa |
WB
Various lysates were subjected to SDS PAGE followed by western blot with Aconitase 1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB
Various lysates were subjected to SDS PAGE followed by western blot with Aconitase 1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using Aconitase 1 antibody at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Aconitase 1 antibody at dilution of 1:200 and Omnimabs®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-Aconitase 1 (IP: 4ug; Detection: 1:5000) with HepG2 cells lysate 1925 ug.| Application Notes | WB:1:1000-1:4000 IHC:1:200-1:800 IF-P:1:50-1:500 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
