Application
Western Blot at 1:2000 dilution Lane 1: human kidney lysate Lane 2: human liver lysate Lane 3: U-2OS whole cell lysate Lane 4: A431 whole cell lysate Lane 5: mouse kidney lysate Lysates/proteins at 20 ug per lane.| Product Name | ACO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding.1) Hirling H.,et al.Nucleic Acids Res. 20:33-39(1992). |
| Immunogen | This ACO1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 354-388 amino acids from the Central region of human ACO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACO1 |
|---|---|
| Alternative Names | Cytoplasmic aconitate hydratase Aconitase 4.2.1.3 Citrate hydro-lyase Ferritin repressor protein Iron regulatory protein 1 IRP1 Iron-responsive element-binding protein 1 IRE-BP 1 ACO1 IREB1 |
| Molecular Weight(MW) | 98 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western Blot at 1:2000 dilution Lane 1: human kidney lysate Lane 2: human liver lysate Lane 3: U-2OS whole cell lysate Lane 4: A431 whole cell lysate Lane 5: mouse kidney lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
