Application
Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 μg/mL.Application
Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/mL.| Product Name | ADAM10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ADAM10 Antibody: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta. The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively. ADAM10 mRNA is expressed in a variety of human and bovine tissues.1) Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis factor-α-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93 |
| Immunogen | ADAM10 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ADAM10. The immunogen is located within the last 50 amino acids of ADAM10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ADAM10 can be used for detection of ADAM10 by Western blot at 1 - 2 μg/mL. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAM10 |
|---|---|
| Alternative Names | ADAM10 Antibody: RAK kuz AD10 AD18 MADM CD156c HsT18717 KUZ Disintegrin and metalloproteinase domain-containing protein 10 CDw156 ADAM 10 |
| Molecular Weight(MW) | 85 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ADAM10 in K562 cells with ADAM10 antibody at 10 μg/mL.
Application
Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/mL.| Application Notes | ADAM10 can be used for detection of ADAM10 by Western blot at 1 - 2 μg/mL. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL. |
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| Form | Liquid |
|---|---|
| Storage Instructions | ADAM10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ADAM10 Antibody is supplied in PBS containing 0.02% sodium azide. |
