WB
Western blot analysis of lysates from Rat kidney using ADAM10 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.ICC/IF
Immunofluorescence analysis of HCT 116 cells using ADAM10 Rabbit pAb at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining| Product Name | ADAM10 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Protein corresponding to a sequence within amino acids 214-672 of human ADAM10(NP_001101.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000-1:5000 ICC:1:50-1:200 FC:1:500-1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAM10 |
|---|---|
| Gene Synonyms | RAK kuz AD10 AD18 MADM CD156c CDw156 HsT18717 |
| Gene Full Name | ADAM metallopeptidase domain 10 |
| Gene Summary | Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016] |
| Molecular Weight(MW) | 84kDa（Observed:68 kDa） |
| Cellular Localization | Cell membrane,Endomembrane system,Single-pass type I membrane protein |
WB
Western blot analysis of lysates from Rat kidney using ADAM10 Rabbit pAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
ICC/IF
Immunofluorescence analysis of HCT 116 cells using ADAM10 Rabbit pAb at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)at 1:500 dilution. Blue: DAPI for nuclear staining| Application Notes | WB:1:1000-1:5000 ICC:1:50-1:200 FC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
