Application
Western blot analysis of PRAS40 (pT246) expression in LO2 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates.WB
e Effects of LY294002 (a PI3K inhibitor) or AKT siRNA on the levels of total AKT, pAKT, phosphorylated PRAS40 (Proline-rich AKT substrate 40 kDa) and pMDM2 were examined by Western blot analysis in MCF-7 cells when HBXIP was overexpressed. f Effects of MK-2206 (an AKT inhibitor) or HBXIP siRNA on the levels of total AKT, pAKT, pPRAS40, and pMDM2 were examined by Western blot analysis in MCF-7-HBXIP cells.| Product Name | PRAS40 (phospho Thr246) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRAS40 (phospho Thr246)AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PRAS40 (pT246). The exact sequence is proprietary. |
| Modification | p-Thr246 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AKT1 substrate 1 (proline-rich) AKT1S1 PRAS40 Lobe |
|---|---|
| Target | PRAS40 |
| Tissue Specificity | Recognizes endogenous levels of PRAS40 (pT246) protein. |
Application
Western blot analysis of PRAS40 (pT246) expression in LO2 (A); Raw264.7 (B); NIH3T3 (C) whole cell lysates.
WB
e Effects of LY294002 (a PI3K inhibitor) or AKT siRNA on the levels of total AKT, pAKT, phosphorylated PRAS40 (Proline-rich AKT substrate 40 kDa) and pMDM2 were examined by Western blot analysis in MCF-7 cells when HBXIP was overexpressed. f Effects of MK-2206 (an AKT inhibitor) or HBXIP siRNA on the levels of total AKT, pAKT, pPRAS40, and pMDM2 were examined by Western blot analysis in MCF-7-HBXIP cells.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
