Application
Immunofluorescence analysis of methanol-fixed HeLa, using Presenilin 1 antibody at 1:200 dilution.Application
Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.Application
Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Presenilin 1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Presenilin 1 (presenilin 1)Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AD3 antibody FAD antibody PS1 antibody S182 antibody PSEN1 antibody presenilin-1 antibody presenilin 1 antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cell surface |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Presenilin 1 antibody at 1:200 dilution.
Application
Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.
Application
Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Presenilin 1 antibody [C1C2], Internal dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
