| Product Name | PRG2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1.B.787] to PRG2PRG2 is a cytotoxin and helminthotoxin. Also induces noncytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA. In pregnancy serum, the proform exists as a disulfide-linked 2:2 heterotetramer with PAPPA, as a disulfide-linked 2:2 heterotetramer with AGT, and as a complex (probably a 2:2:2 heterohexamer) with AGT and C3dg. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1.B.787 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-FIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | BMPG MBP MBP1 MGC14537 |
|---|---|
| Target | PRG2 |
| Tissue Specificity | Specific for eosinophil major basic protein (14kD) irrespective of the stages of eosinophil activation and can be regarded as a ?pan-eosinophil? marker. Recognizes secreted pro-form of MBP (Pregnancy associated MBP, 23.8kD) and mature form found in the matrix of the eosinophil large specific granule (13.8kD). |
| Cellular Localization | The proform is secreted. The mature protein is found in the matrix of the eosinophil's large specific granule (crystalloid core). |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IHC-Fr: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 26.6 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Aliquots are stable for 12 months. |
| Storage Buffer | Preservative: 10mM Sodium Azide; Constituents: 1mg/ml BSA, PBS. pH 7.2 |
