| Product Name | PRKAR2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [RS6] to PRKAR2cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). |
| Immunogen | Purified regulatory subunit of cAMP-dependent protein kinase type II from bovine brain. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | RS6 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IF |
| ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanPigBovine |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | cAMP dependent protein kinase regulatory subunit RII alpha PKR2 PRKAR Protein kinase A RII-alpha subunit Protein kinase cAMP-dependent regulatory type II alpha |
|---|---|
| Target | PRKAR2 |
| Tissue Specificity | Strongly inhibits holoenzyme formation and cAMP binding. |
| Cellular Localization | Cell Membrane |
| Application Notes | ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
