Application
Flow cytometric Analysis of Hela cells, using anti-PRKD2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC215335 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.| Product Name | PRKD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3A1] to PRKD2Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress. |
| Immunogen | Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 3A1 |
| Host Species | Mouse |
| Tested Applications | FACSWB |
| ICC/IF 1:100 : |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKD2 PRKD2 HSPC187 |
|---|---|
| Target | PRKD2 |
| Tissue Specificity | is specific for human PRKD2 (NP_057541). |
| Cellular Localization | Translocation to the cell membrane is required for kinase activation. |
Application
Flow cytometric Analysis of Hela cells, using anti-PRKD2 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC215335 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKD2 antibody, and then analyzed by flow cytometry.
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2.| Application Notes | ICC/IF 1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
