Application
Flow cytometric Analysis of Jurkat cells, using anti-PRKG1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).Application
HEK293T cells transfected with either RC220986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKG1 antibody, and then analyzed by flow cytometry.Application
Anti-PRKG1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PRKG1Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1.| Product Name | PRKG1 antibody [9A4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [9A4] to PRKG1Phosphorylates PPP1R12A. |
| Immunogen | Full length human recombinant protein of human PRKG1 (NP_006249) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 9A4 |
| Host Species | Mouse |
| Tested Applications | FACS,ICC/IF,WB |
| IHC 1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CGKI, cGKIalpha, PRKG1B, cGKIBETA, PGK, cGKI-alpha, MGC71944, PRKG1, cGKI-BETA, PRKGR1B, cGKI BETA, cGKI alpha |
|---|---|
| Target | PRKG1 |
| Tissue Specificity | is specific for human PRKG1 (NP_006249). |
Application
Flow cytometric Analysis of Jurkat cells, using anti-PRKG1 antibody,(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Application
HEK293T cells transfected with either RC220986 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKG1 antibody, and then analyzed by flow cytometry.
Application
Anti-PRKG1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with PRKG1
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKG1.| Application Notes | IHC 1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
