Application
Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3| Product Name | PRMT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [PRMT1 - 171] to PRMT1Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins (Histone 2A, 3, 4), IL enhancer binding factor, nuclear factors (NF45, 90, ILF3, Nucleolin, STAT1, Poly(A) binding protein II), cell cycle proteins (phosphoprotein phosphatase 2A), signal transduction proteins (FGF2, Fibrillarin, FN, INFAR1, Jak, MBP, Src adaptor Sam68), apoptosis proteins (FADD, ICE like protease), and viral proteins (Hepatitis C NS3 RNA Helicase, HIV TAR). The mammalian PRMT family currently consists of 5 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all five PRMT members have been developed. These antibodies can be utilized to explore arginine methylation and its regulatory functions. |
| Immunogen | Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | PRMT1-171 |
| Host Species | Mouse |
| Tested Applications | DIDELISAIPWB |
| DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 μg/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogMonkeyRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 6720434D09Rik AW214366 Hrmt1l2 Mrmt1 |
|---|---|
| Target | PRMT1 |
| Tissue Specificity | This antibody reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5. |
| Cellular Localization | Nuclear |
Application
Cell line lysates were separated on SDS-PAGE and probed with 2 μg/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3| Positive Control | Human kidney cell line 293T |
|---|---|
| Application Notes | DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 μg/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
