Application
PRMT5 antibody [GT3610] immunoprecipitates PRMT5 protein in IP experiments. IP samples: Raji whole cell extract A. 35 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [GT3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [GT3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
PRMT5 antibody detects PRMT5 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE PRMT5 antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
PRMT5 antibody [GT3610] detects PRMT5 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 7.5% SDS-PAGE PRMT5 antibody [GT3610] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | PRMT5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to PRMT5 (protein arginine methyltransferase 5) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRMT5. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT3610 |
| Host Species | Mouse |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Japanese medaka, Xenopus laevis, Pig, Rhesus Monkey, Chimpanzee, BovineMouse (98%), Zebrafish (85%), Japanese medaka (86%), Xenopus laevis (91%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 73 kDa (note) (7) |
|---|---|
| Cellular Localization | Cytoplasm , Nucleus |
Application
PRMT5 antibody [GT3610] immunoprecipitates PRMT5 protein in IP experiments. IP samples: Raji whole cell extract A. 35 μg Raji whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of PRMT5 protein by 4 μg PRMT5 antibody [GT3610] 7.5 % SDS-PAGE The immunoprecipitated PRMT5 protein was detected by PRMT5 antibody [GT3610] diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
PRMT5 antibody detects PRMT5 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg HL-60 whole cell lysate/extract F. 30 µg NCI-H929 whole cell lysate/extract 7.5% SDS-PAGE PRMT5 antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
PRMT5 antibody [GT3610] detects PRMT5 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 7.5% SDS-PAGE PRMT5 antibody [GT3610] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , K562 , THP-1 , HL-60 , NCI-H929 , rat kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
