Application
Detection of human recombinant proBNP expressed mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody [5B6] ; Lane 2: proBNP antibody [15F11] ; Lane 3: proBNP antibody [3G12] ; Lane 4: proBNP antibody [11D1] ; Lane 5: proBNP antibody [24E11] ; Both proBNP antibody [11D1] and proBNP antibody [24E11] do not recognize glycosylated proBNP expressed in mammalian cells.| Product Name | proBNP antibody [13G12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [13G12] to proBNPIn cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa ""signal"" peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure. |
| Immunogen | Synthetic peptide: ETSGLQEQRNHLQGK, corresponding to N terminal amino acids 13-27 of Human proBNP. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 13G12 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| For ELISA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | proBNP, P16860, 600295, BNP, NPPB, 4879 |
|---|---|
| Target | proBNP |
| Cellular Localization | Secreted |
Application
Detection of human recombinant proBNP expressed mammalian cells in Western blotting by different monoclonal antibodies after Tricine-SDS gel electrophoresis. Antigen: recombinant proBNP expressed in mammalian cells, 0.15 ug; Lane 1: proBNP antibody [5B6] ; Lane 2: proBNP antibody [15F11] ; Lane 3: proBNP antibody [3G12] ; Lane 4: proBNP antibody [11D1] ; Lane 5: proBNP antibody [24E11] ; Both proBNP antibody [11D1] and proBNP antibody [24E11] do not recognize glycosylated proBNP expressed in mammalian cells.| Application Notes | For ELISA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate buffered saline, pH7.4, containing 0.1% sodium azide |
