Application
Confocal immunofluorescent analysis of AGL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of hAGL-C371 in K562 cell line lysates (35ug/lane)| Product Name | AGL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE |
| Product description | AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.1) Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002). |
| Immunogen | This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-387 amino acids from the Central region of human AGL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AGL |
|---|---|
| Alternative Names | Glycogen debranching enzyme Glycogen debrancher 4-alpha-glucanotransferase Oligo-1 4-1 4-glucantransferase Amylo-alpha-1 6-glucosidase Amylo-1 6-glucosidase Dextrin 6-alpha-D-glucosidase AGL GDE |
| Molecular Weight(MW) | 175 kDa |
Application
Confocal immunofluorescent analysis of AGL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of hAGL-C371 in K562 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
