Application
Detection of human recombinant PCT by monoclonal anti- bodies specific to N-terminal fragment of PCT after 15% SDS-PAGE in reducing conditions. Antigen loaded - 100 ng/track. 1 – antibody [6F10] 2 – antibody [27A3] 3 – antibody [44d9]Application
Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.| Product Name | Procalcitonin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [44D9] to ProcalcitoninProcalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. The amino acid sequence of PCT was firstly described by Moullec et al. in 1984. It belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 a.a.r. from N-terminus. PCT is produced normally in C-cells of the thyroid glands. It undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.r.), calcitonin (32 a.a.r.) and katacalcin (21 a.a.r.). It has been shown that the level of PCT in serum increases significantly during an infection of bacterial origin (Assicot M, et al). Today PCT is considered to be one of the earliest and most specific markers of sepsis. However, several studies revealed that elevated PCT level in human blood could be detected not only in case of sepsis and infection, but also in cases of surgery, polytrauma, heat shock and cardiogenic shock (Meisner M. & Reinhart K). The importance of PCT measurements in combination with cTnT or cTnI during heart transplantation to predict an early graft failure has been proved(Potapov EV., et al). |
| Immunogen | Native protein N-terminal fragment (purified) (Human) (see figure) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 44D9 |
| Host Species | Mouse |
| Tested Applications | EIAELISAWB |
| ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 13 kDa. Can be paired for ELISA with GTX11493 Mouse monoclonal [16B5 ] to Calcitonin . Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | CALC1 CGRP CGRP-I CGRP1 CT KC MGC126648 CGRP I CGRPI |
|---|---|
| Target | Procalcitonin |
Application
Detection of human recombinant PCT by monoclonal anti- bodies specific to N-terminal fragment of PCT after 15% SDS-PAGE in reducing conditions. Antigen loaded - 100 ng/track. 1 – antibody [6F10] 2 – antibody [27A3] 3 – antibody [44d9]
Application
Amino acid sequence and schematic diagram of human procalcitonin and the N-terminal, calcitonin, and katacalcin (catacalcin) fragments.| Application Notes | ELISA: Use at an assay dependent dilution. EIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 13 kDa. Can be paired for ELISA with GTX11493 Mouse monoclonal [16B5 ] to Calcitonin . Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Phosphate-buffered saline, pH7.4, containing 0.1% sodium azide |
