Application
Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.Application
Immunofluorescence of AID in Ramos cells with AID antibody at 20 μg/mL.Application
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/mL.| Product Name | AID Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AID Antibody: AID, ARP2, CDA2, HIGM2, HEL-S-284, AID, Single-stranded DNA cytosine deaminase, Activation-induced cytidine deaminase |
| Product description | AID Antibody: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G.1) Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6. |
| Immunogen | AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human AID. The immunogen is located within the last 50 amino acids of AID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AICDA |
|---|---|
| Alternative Names | AID Antibody: AID ARP2 CDA2 HIGM2 HEL-S-284 AID Single-stranded DNA cytosine deaminase Activation-induced cytidine deaminase |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/mL in either the (A) absence or (B) presence of blocking peptide.
Application
Immunofluorescence of AID in Ramos cells with AID antibody at 20 μg/mL.
Application
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1225 - Ramos Cell Lysate |
|---|---|
| Application Notes | AID antibody can be used for detection of AID by Western blot at 2 μg/mL. Anti-AID is human and mouse reactive. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AID antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AID Antibody is supplied in PBS containing 0.02% sodium azide. |
