| Product Name | Progesterone Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [4D8] to Progesterone receptorThe human Progesterone Receptor (PgR) is a member of the steroid family of nuclear receptors. PgR is found as a 120 kDa protein (Form B) or a 94 kDa protein (Form A) due to the use of alternative translation initiation sites. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. When bound to DNA, PgR can positively or negatively regulate gene transcription through the recruitment of coactivator (e.g. SRC-1) or corepressor (e.g. NCoR) proteins. |
| Immunogen | 6-His fusion protein containing the region encoding amino acids 1-101 of the human progesterone receptor expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 4D8 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 99 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | progesterone receptor PgR Anti-NR3C3 Anti-PGR Anti-PR Anti-Progesterone Receptor NR3C3 antibody PGR antibody PR antibody |
|---|---|
| Target | Progesterone receptor |
| Tissue Specificity | This antibody recognizes both Form A (94 kDa) and Form B (120kDa) of the human progesterone receptor (PgR) |
| Cellular Localization | Nuclear |
| Light Chain Type | kappa |
| Positive Control | MCF7 lysate (GTX23871) & recombinant protein |
|---|---|
| Application Notes | For WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 99 kDa. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing no preservatives |
