Application
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:1000| Product Name | Prohibitin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Prohibitin (prohibitin)Prohibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Prohibitin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Cat, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (94%), Xenopus laevis (89%), Dog (100%), Cat (100%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PHB1 antibody PHB antibody prohibitin antibody |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (5) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Prohibitin antibody detects Prohibitin protein at mitochondria by immunofluorescent analysis. Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Prohibitin protein stained by Prohibitin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
Application
Prohibitin antibody detects PHB protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg GL261 whole cell lysate/extract C. 30 µg C8D30 whole cell lysate/extract 12 % SDS-PAGE Prohibitin antibody dilution: 1:1000
Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Prohibitin antibody dilution: 1:500.
Application
Prohibitin antibody detects Prohibitin protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Prohibitin antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using Prohibitin antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 12% SDS PAGE diluted at 1:1000| Positive Control | IMR32 , U87-MG , Neuro2A , GL261 , C8D30 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
