Application
ELISA analysis of control antigen and antigen using PHB antibody [5H7].Application
FACS analysis of MCF-7 cells using PHB antibody [5H7] (blue) or a negative control (red).Application
ICC/IF analysis of NIH3T3 cells using PHB antibody [5H7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).Application
IHC-P analysis of liver cancer tissues using PHB antibody [5H7].Application
IHC-P analysis of lung cancer tissues using PHB antibody [5H7].Application
IHC-P analysis of rectum cancer tissues using PHB antibody [5H7].Application
IHC-P analysis of stomach cancer tissues using PHB antibody [5H7].Application
WB analysis of (1) A431, (2) MCF-7, (3) Jurkat, (4) HeLa, (5) HepG2, (6) A549, (7) NIH3T3, (8) Cos7 and (9) PC-12 lysates using PHB antibody [5H7].Application
WB analysis of human PHB (a.a. 68-259) recombinant protein using PHB antibody [5H7].| Product Name | Prohibitin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [5H7] to ProhibitinProhibitin is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human PHB expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5H7 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | PHB1 |
|---|---|
| Target | PHB |
| Molecular Weight(MW) | 30 kDa (note) |
Application
ELISA analysis of control antigen and antigen using PHB antibody [5H7].
Application
FACS analysis of MCF-7 cells using PHB antibody [5H7] (blue) or a negative control (red).
Application
ICC/IF analysis of NIH3T3 cells using PHB antibody [5H7] (green), Alexa Fluor-555 phalloidin (red) and DRAQ5 fluorescent DNA dye (blue).
Application
IHC-P analysis of liver cancer tissues using PHB antibody [5H7].
Application
IHC-P analysis of lung cancer tissues using PHB antibody [5H7].
Application
IHC-P analysis of rectum cancer tissues using PHB antibody [5H7].
Application
IHC-P analysis of stomach cancer tissues using PHB antibody [5H7].
Application
WB analysis of (1) A431, (2) MCF-7, (3) Jurkat, (4) HeLa, (5) HepG2, (6) A549, (7) NIH3T3, (8) Cos7 and (9) PC-12 lysates using PHB antibody [5H7].
Application
WB analysis of human PHB (a.a. 68-259) recombinant protein using PHB antibody [5H7].| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
