Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-473).Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using Akt (Ab-473).| Product Name | Akt Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, MEK1, PRKMK1, AKT, C-AKT, PKB-alpha |
| Product description | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.1) Baudhuin LM, et al. (2004) FASEB J Feb; 18 (2): 341-3. Min YH, et al. (2004) Cancer Res; 64 (15): 5225-31. Feng J, et al. (2004) J Biol Chem; 279 (34): 35510-7. Ayala G, et al. (2004) Clin Cancer Res; 10 (19): 6572-8. Lungu AO, et al. (2004) J Biol Chem; 279 (47): 48794-800. |
| Immunogen | Akt (Ab-473) antibody was raised against a peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA MEK1 PRKMK1 AKT C-AKT PKB-alpha |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Akt protein. |
| Entrez Gene | 207 |
|---|---|
| Protein Accession | NP_001014431.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Ab-473).
Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using Akt (Ab-473).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM270692