Application
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.Application
Antibody used in WB on Xenopus laevis embryos at: 1:500 (Lanes: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer)).Application
Antibody used in WB on baboon muscle homogenate at 1:1666 (Lane 1: 50ug preterm baboon muscle homogenate Lane 2: 50ug preterm baboon muscle homogenate Lane 3: 50ug term baboon muscle homogenate Lane 4: 50ug term baboon muscle homogenate Lane 5: 50ug adult baboon muscle homogenate Lane 6: 50ug adult baboon muscle homogenate ).| Product Name | AKT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AKT1, AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA |
| Product description | AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.1) Kim, H.A., et al., (2006) Exp. Mol. Pathol. 80 (2), 165-170. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AKT1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AKT1 |
|---|---|
| Alternative Names | AKT1 AKT PKB RAC CWS6 PRKBA PKB-ALPHA RAC-ALPHA |
| Molecular Weight(MW) | 56 kDa |
Application
Antibody used in WB on Human Small Intestine at 0.2-1 ug/ml.
Application
Antibody used in WB on Xenopus laevis embryos at: 1:500 (Lanes: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer)).
Application
Antibody used in WB on baboon muscle homogenate at 1:1666 (Lane 1: 50ug preterm baboon muscle homogenate Lane 2: 50ug preterm baboon muscle homogenate Lane 3: 50ug term baboon muscle homogenate Lane 4: 50ug term baboon muscle homogenate Lane 5: 50ug adult baboon muscle homogenate Lane 6: 50ug adult baboon muscle homogenate ).| Positive Control | 1) Cat. No. XBL-10420 - Fetal Small Intestine Tissue Lysate |
|---|---|
| Application Notes | AKT1 antibody can be used for detection of AKT1 by ELISA at 1:312500. AKT1 antibody can be used for detection of AKT1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AKT1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
Data sheet for OM270713