Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6 antibody at 1:200 dilution.Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000| Product Name | Proteasome 20S alpha 6 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S alpha 6 (proteasome (prosome, macropain) subunit, alpha type, 6)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (100%), Xenopus laevis (89%), Pig (99%), Chicken (96%), Sheep (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IOTA antibody MGC22756 antibody MGC2333 antibody MGC23846 antibody PROS27 antibody p27K antibody PSMA6 antibody 27 kDa prosomal protein antibody multicatalytic endopeptidase complex iota chain antibody macropain iota chain antibody prosomal P27K |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 20S Proteasome alpha 6 antibody at 1:200 dilution.
Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
