Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Proteasome 20S alpha 6 antibody dilution: 1:500.Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody dilution: 1:500.Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Proteasome 20S alpha 6 antibody dilution: 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 12% SDS PAGE diluted at 1:1000Application
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000Application
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 µg rat liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000| Product Name | Proteasome 20S alpha 6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Proteasome 20S alpha 6 (proteasome (prosome, macropain) subunit, alpha type, 6)The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineXenopus laevis (87%), Pig (98%), Chicken (96%), Sheep (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IOTA antibody MGC22756 antibody MGC2333 antibody MGC23846 antibody PROS27 antibody p27K antibody PSMA6 antibody 27 kDa prosomal protein antibody multicatalytic endopeptidase complex iota chain antibody macropain iota chain antibody prosomal P27K |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Proteasome 20S alpha 6 antibody dilution: 1:500.
Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody dilution: 1:500.
Application
Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Proteasome 20S alpha 6 antibody dilution: 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using 20S Proteasome alpha6 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft , using 20S Proteasome alpha6 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 12% SDS PAGE diluted at 1:1000
Application
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000
Application
Proteasome 20S alpha 6 antibody detects PSMA6 protein by Western blot analysis. A. 50 µg rat liver lysate/extract 12 % SDS-PAGE Proteasome 20S alpha 6 antibody dilution: 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
