ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ALDH4A1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
ALDH4A1 antibody can be used for detection of ALDH4A1 by ELISA at 1:62500. ALDH4A1 antibody can be used for detection of ALDH4A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 cells at 2.5 ug/ml.
Application
Antibody used in IHC on Human Intestine.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
ALDH4A1 antibody can be used for detection of ALDH4A1 by ELISA at 1:62500. ALDH4A1 antibody can be used for detection of ALDH4A1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ALDH4A1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt