Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-ALDH4A1 Antibody in HepG2 cell line lysates (35ug/lane).Application
Western blot analysis of ALDH4A1 using rabbit polyclonal ALDH4A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH4A1 gene.| Product Name | ALDH4A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial, P5C dehydrogenase, Aldehyde dehydrogenase family 4 member A1, L-glutamate gamma-semialdehyde dehydrogenase, ALDH4A1, ALDH4, P5CDH |
| Product description | ALDH4A1 belongs to the aldehyde dehydrogenase family of proteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associated with type II hyperprolinemia, an autosomal recessive disorder characterized by accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline.1) Yoon,K.A., J. Hum. Genet. 49 (3), 134-140 (2004) |
| Immunogen | This ALDH4A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-314 amino acids from the Central region of human ALDH4A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ALDH4A1 |
|---|---|
| Alternative Names | Delta-1-pyrroline-5-carboxylate dehydrogenase mitochondrial P5C dehydrogenase Aldehyde dehydrogenase family 4 member A1 L-glutamate gamma-semialdehyde dehydrogenase ALDH4A1 ALDH4 P5CDH |
| Molecular Weight(MW) | 62 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH4A1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-ALDH4A1 Antibody in HepG2 cell line lysates (35ug/lane).
Application
Western blot analysis of ALDH4A1 using rabbit polyclonal ALDH4A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ALDH4A1 gene.| Application Notes | For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
