Application
Western blot analysis of extracts from COS7 cells treated with Anisomycin using Phospho-ALK (Tyr1507) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | ALK (phospho Tyr1507) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | kinase ALK, Anaplastic lymphoma kinase, CD246, ALK tyrosine kinase receptor precursor, |
| Product description | Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. PTN-binding induces MAPK pathway activation, which is important for the anti-apoptotic signaling of PTN and regulation of cell proliferation. MDK-binding induces phosphorylation of the ALK target insulin receptor substrate (IRS1), activates mitogen-activated protein kinases (MAPKs) and PI3-kinase, resulting also in cell proliferation induction. Drives NF-kappa-B activation, probably through IRS1 and the activation of the AKT serine/threonine kinase. Recruitment of IRS1 to activated ALK and the activation of NF-kappa-B are essential for the autocrine growth and survival signaling of MDK.1) Degoutin J, Vigny M, Gouzi JY (2007) FEBS Lett 581, 727-34. |
| Immunogen | ALK (phospho Tyr1507) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 1507 (P-T-Y (p)-G-S) derived from Human ALK. |
| Modification | p-Tyr1507 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Alk |
|---|---|
| Alternative Names | kinase ALK Anaplastic lymphoma kinase CD246 ALK tyrosine kinase receptor precursor |
| Molecular Weight(MW) | 176 kDa |
| Tissue Specificity | The antibody detects endogenous level of ALK only when phosphorylated at tyrosine 1507. |
| Entrez Gene | 238 |
|---|---|
| Protein Accession | NP_004295.2 |
Application
Western blot analysis of extracts from COS7 cells treated with Anisomycin using Phospho-ALK (Tyr1507) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
