Application
AMACR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of AMACR Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | AMACR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA racemase, AMACR |
| Product description | This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described.1) Murray, N.P., et al. Oncol. Rep. 24(3):687-692(2010) Sonwalkar, S.A., et al. Histopathology 56(7):900-907(2010) Lakis, S., et al. World J. Gastroenterol. 16(20):2476-2483(2010) Chen, W., et al. Mol. Biol. Rep. 36(3):423-430(2009) Mubiru, J.N., et al. Gene 327(1):89-98(2004) |
| Immunogen | This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AMACR |
|---|---|
| Alternative Names | Alpha-methylacyl-CoA racemase 2-methylacyl-CoA racemase AMACR |
| Molecular Weight(MW) | 42 kDa |
Application
AMACR antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of AMACR Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution Lane 1: human kidney lysate Lane 2: LNCaP whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
