Application
Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.Application
Immunohistochemical analysis of paraffin - embedded human prostate adenocarcinoma tissue showing cytoplasma location using AMACR antibody with DAB staining.| Product Name | AMACR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 2-methylacyl-CoA racemaseRM, RACE, CBAS4, AMACRD |
| Product description | AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.1) Chen Q. Watson JT. Marengo SR. et al. Cancer Lett. 2006, Dec 8, 244 (2):274-88.Epub 2006 Feb 23. |
| Immunogen | Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AMACR |
|---|---|
| Alternative Names | 2-methylacyl-CoA racemaseRM RACE CBAS4 AMACRD |
Application
Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.
Application
Immunohistochemical analysis of paraffin - embedded human prostate adenocarcinoma tissue showing cytoplasma location using AMACR antibody with DAB staining.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AMACR monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
