Application
Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | AML1 (phospho Ser435) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acute myeloid leukemia 1 protein, CBFA2, CBFA2, alpha 2 subunit, RUNX1 |
| Product description | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.1) Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991). |
| Immunogen | AML1 (phospho Ser435) antibody was raised against a peptide sequence around phosphorylation site of Serine 435 ( S-N-S (p)-P-T) derived from Human AML1. |
| Modification | p-Ser435 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUNX1 |
|---|---|
| Alternative Names | Acute myeloid leukemia 1 protein CBFA2 CBFA2 alpha 2 subunit RUNX1 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | The antibody detects endogenous levels of AML1 only when phosphorylated at serine 435. |
| Entrez Gene | 861 |
|---|---|
| Protein Accession | NP_001001890.1 |
Application
Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
