Application
Western blot analysis of extract from P19, HeLa and 293 cells using AML1 (RUNX1).| Product Name | AML1 (RUNX1) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AML1, CBFA2, EVI-1, AMLCR1, PEBP2aB, AML1-EVI-1 |
| Product description | CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits MYST4-dependent transcriptional activation.1) Mao S., Frank R. C., Zhang J., Miyazaki Y., Nimer S. D. Mol. Cell. Biol. 19:3635-3644 (1999) Pelletier N., Champagne N., Stifani S., Yang X. -J. Oncogene 21:2729-2740 (2002) Cho J. -Y., Akbarali Y., Zerbini L. F., Gu X., Boltax J., Wang Y., Oettgen P., Zhang D. -E., Libermann T. A. J. Biol. Chem. 279:19512-19522 (2004) |
| Immunogen | AML1 (RUNX1) antibody was raised against a peptide sequence around aa.6~10 (D-A-S-T-S) derived from Human AML1 (RUNX1). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RUNX1 |
|---|---|
| Alternative Names | AML1 CBFA2 EVI-1 AMLCR1 PEBP2aB AML1-EVI-1 |
| Molecular Weight(MW) | 55 kDa |
| Tissue Specificity | This antibody detects endogenous level of total AML1(RUNX1) protein. |
| Entrez Gene | 861 |
|---|---|
| Protein Accession | NP_001116079.1 |
Application
Western blot analysis of extract from P19, HeLa and 293 cells using AML1 (RUNX1).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
