Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | AMPK alpha 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1 |
| Product description | AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK.1) Zhang, Q.H., et al., Genome Res. 10(10):1546-1560 (2000). |
| Immunogen | This AMPK alpha 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-510 amino acids from the C-terminal region of human AMPK alpha 1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:8000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRKAA1 |
|---|---|
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-1 AMPK subunit alpha-1 Acetyl-CoA carboxylase kinase ACACA kinase Hydroxymethylglutaryl-CoA reductase kinase HMGCR kinase Tau-protein kinase PRKAA1 PRKAA1 AMPK1 |
| Molecular Weight(MW) | 64 kDa |
Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:8000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM271026