Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35ug/lane)Application
PRKAA1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | PRKAA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1 |
| Product description | PRKAA1 is a protein belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase. PRKAA1 is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of PRKAA1 is activated by the stimuli that increase the cellular AMP/ATP ratio. PRKAA1 regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.1) Puissant,A., et.al., Cancer Res. 70 (3), 1042-1052 (2010) |
| Immunogen | This PRKAA1 antibody is generated from rabbits immunized with a recombinant protein from human PRKAA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 |
|---|---|
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-1 AMPK subunit alpha-1 Acetyl-CoA carboxylase kinase ACACA kinase Hydroxymethylglutaryl-CoA reductase kinase HMGCR kinase Tau-protein kinase PRKAA1 PRKAA1 AMPK1 |
| Molecular Weight(MW) | 64 kDa |
Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PRKAA1 Antibody in K562, MDA-MB435 cell line lysates (35ug/lane)
Application
PRKAA1 Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM288806