Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser487). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1(Phospho-Ser487)Antibody #11174.Application
Western blot analysis of lysed extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser487) Antibody.| Product Name | AMPKa1 (phospho Ser487) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMPK, AMPKa1, FLK1, VEGFR2, AAPK1, AMPK alpha-1 chain, AMPK-alpha1, HMG-CoA redustase kinase, PRKAA1 |
| Product description | Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.1) Kim JE, et al. (2005) J Proteome Res. 4 (4): 1339-1346. Woods A, et al. (2003) J Biol Chem. 278 (31): 28434-28442. |
| Immunogen | AMPKa1 (Phospho-Ser487) antibody was raised in was raised against a peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKα1. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 |
|---|---|
| Alternative Names | AMPK AMPKa1 FLK1 VEGFR2 AAPK1 AMPK alpha-1 chain AMPK-alpha1 HMG-CoA redustase kinase PRKAA1 |
| Molecular Weight(MW) | 63 kDa |
| Tissue Specificity | This antibody detects endogenous level of AMPKα1 only when phosphorylated at serine 487. |
| Entrez Gene | 5562 |
|---|---|
| Protein Accession | NP_006242.5 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser487). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1(Phospho-Ser487)Antibody #11174.
Application
Western blot analysis of lysed extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser487) Antibody.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM271034