Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demondetected the ANDR protein (arrow).Application
Androgen Receptor Antibody (ANDR) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Androgen Receptor Antibody (ANDR) for immunohistochemistry.| Product Name | Androgen Receptor Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4 |
| Product description | Androgen receptor (ANDR) has 3 major functional domains: the N-terminal domain, DNA-binding domain, and an androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The gene for this protein contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of the protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations are also associated with complete androgen insensitivity (CAIS). PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor; sumoylation of ANDR represses androgen receptor dependent transcription.1) Nishida, et al. J. Biol. Chem. 277 (44), 41311-41317 (2002) |
| Immunogen | This Androgen Receptor (ANDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-392 amino acids from human Androgen Receptor (ANDR). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AR |
|---|---|
| Alternative Names | Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 |
| Molecular Weight(MW) | 99 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).
Application
Western blot analysis in Hela cell line lysates (35ug/lane).This demondetected the ANDR protein (arrow).
Application
Androgen Receptor Antibody (ANDR) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Androgen Receptor Antibody (ANDR) for immunohistochemistry.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
