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ELISA: (direct or indirect: 1:500-1:5,000). Western blot: (1:500-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions should be determined individually for each application.:
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