Application
Antibody used in WB on Human Placenta at 2.5 ug/ml.| Product Name | ANXA7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANXA7, SNX, ANX7, SYNEXIN |
| Product description | ANXA7 is a member of the annexin family of calcium-dependent phospholipid binding proteins. The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly (A) signals. The selection of poly (A) signals is independent of the mRNA splicing pattern. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.1) Satoh, H., et al., (2002) Biochim. Biophys. Acta 1600 (1-2), 61-67. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA7. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ANXA7 antibody can be used for detection of ANXA7 by ELISA at 1:312500. ANXA7 antibody can be used for detection of ANXA7 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANXA7 |
|---|---|
| Alternative Names | ANXA7 SNX ANX7 SYNEXIN |
| Molecular Weight(MW) | 51 kDa, 53 kDa |
Application
Antibody used in WB on Human Placenta at 2.5 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | ANXA7 antibody can be used for detection of ANXA7 by ELISA at 1:312500. ANXA7 antibody can be used for detection of ANXA7 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
