Application
Western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the detected ANXA7 protein (arrow).Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Immunofluorescence analysis of ANXA7 Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescenApplication
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ANXA7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Annexin A7, Annexin VII, Annexin-7, Synexin, ANXA7, ANX7, SNX |
| Product description | Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. ANXA7 is a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.1) Shirvan,A., et.al., Biochemistry 33 (22), 6888-6901 (1994) |
| Immunogen | This ANXA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the Central region of human ANXA7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANXA7 |
|---|---|
| Alternative Names | Annexin A7 Annexin VII Annexin-7 Synexin ANXA7 ANX7 SNX |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the detected ANXA7 protein (arrow).
Application
(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Immunofluorescence analysis of ANXA7 Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescen
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
