Application
Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of APBA1 in human brain tissue with APBA1 antibody at 20 μg/mL.| Product Name | APBA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APBA1 Antibody: X11, X11A, LIN10, MINT1, D9S411E, X11ALPHA, X11, Amyloid beta A4 precursor protein-binding family A member 1, Adapter protein X11alpha, Mint-1 |
| Product description | APBA1 Antibody: The Amyloid beta 4 (A4) precursor protein-binding family A member 1 (APBA1) is a member of the X11 protein family and a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). APBA1 stabilizes APP and inhibits production of proteolytic APP fragments including the Aβ peptide that is deposited in the brains of Alzheimer's disease patients. It is thought to be involved in signal transduction processes and is a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion. APBA1 can also bind to CASK, a multidomain scaffolding protein involved in brain development and synapse formation.1) Borg JP, Ooi J, Levy E, et al. The phosphotyrosine interaction domains of X11 and FE65 bind to distinct sites on the YENPTY motif of amyloid precursor protein. Mol. Cell. Biol. 1996; 16:6229-41. |
| Immunogen | APBA1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human APBA1. The immunogen is located within amino acids 90 - 140 of APBA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| APBA1 antibody can be used for detection of APBA1 by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APBA1 |
|---|---|
| Alternative Names | APBA1 Antibody: X11 X11A LIN10 MINT1 D9S411E X11ALPHA X11 Amyloid beta A4 precursor protein-binding family A member 1 Adapter protein X11alpha Mint-1 |
| Molecular Weight(MW) | Predicted: 92 kDa Observed: 92 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two alternatively spliced isoforms are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other APBA proteins. |
Application
Western blot analysis of APBA1 in rat brain tissue lysate with APBA1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of APBA1 in human brain tissue with APBA1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | APBA1 antibody can be used for detection of APBA1 by Western blot at 0.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APBA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APBA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
