Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | APIP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Methylthioribulose-1-phosphate dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}, MTRu-1-P dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}, 4.2.1.109 {ECO:0000255|HAMAP-Rule:MF_03116}, APAF1-interacting protein {ECO:0000255|HAMAP-Rule:MF_03116}, hAPIP, APIP {ECO |
| Product description | Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.1) Lai C.-H.,et al.Genome Res. 10:703-713(2000). |
| Immunogen | This APIP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from the Central region of human APIP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APIP |
|---|---|
| Alternative Names | Methylthioribulose-1-phosphate dehydratase {ECO:0000255|HAMAP-Rule:MF_03116} MTRu-1-P dehydratase {ECO:0000255|HAMAP-Rule:MF_03116} 4.2.1.109 {ECO:0000255|HAMAP-Rule:MF_03116} APAF1-interacting protein {ECO:0000255|HAMAP-Rule:MF_03116} hAPIP APIP {ECO |
| Molecular Weight(MW) | 27 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
