Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50–70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-_ agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins.1) Pennacchio, L, Science 2001. 294, 169-173.
Immunogen
Ni-NTA purified recombinant human APOA5 expressed in E. Coli strain BL21 (DE3).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Host Species
Mouse
Tested Applications
ELISAWB
Western Blot, ELISA. Dilution: Western Blot:1:2000 - 1:5,000 ELISA:Determining optimal working dilutions by titration test.:
Western blot analysis using Apoa5 monoclonal antibody against human serum total protein.
Application
Western blot analysis using Apoa5 monoclonal antiobdy against truncated Apoa5 recombinant protein.
Application Notes
Western Blot, ELISA. Dilution: Western Blot:1:2000 - 1:5,000 ELISA:Determining optimal working dilutions by titration test.:
Additional Information
Form
Liquid
Storage Instructions
Apoa5(ab) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer
Purified antibody in PBS containing 0.03% sodium azide.