Application
Immunofluorescense analysis of L - 02 cells showing cytomembrane localization using ApoM.trx antibody.Application
Western blot analysis using ApoM monoclonal antibody against human serum.| Product Name | ApoM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G3A, NG20, HSPC336, Protein G3a, Apo-M, G3a, apo-M |
| Product description | ApoM(apolipoprotein M,also designated G3a or NG20),with 188-amino acid protein(about 21 kDa),is an apolipoprotein and member of the lipocalin protein family.The Apo-proteins are involved in the specific binding of cellular receptors, the regulation of lipolytic enzymes, and the process of lipid exchange. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport.The N-terminal region of Apo-M contains hydrophobic residues that may promote association with the phospholipid layer of lipoprotein particles. In vitro,Apo-M is glycosylated when translated in the presence of microsomes, and remains associated with the microsomes after carbonate treatment.Apo-M is expressed in liver and kidney, and is secreted into the bloodstream in HDLs,and also found in triglyceride-rich lipoproteins and LDLs.1) Xu, N. & Dahlback, B. 1999 J. Biol. Chem. 274:31286. |
| Immunogen | Ni-NTA purified full-length recombinant ApoM.trx expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 IF:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOM |
|---|---|
| Alternative Names | G3A NG20 HSPC336 Protein G3a Apo-M G3a apo-M |
Application
Immunofluorescense analysis of L - 02 cells showing cytomembrane localization using ApoM.trx antibody.
Application
Western blot analysis using ApoM monoclonal antibody against human serum.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IF:1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ApoM monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
