Application
Immunofluorescence staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).Application
Detection of PRR7 / TRAP3 in murine brain lysate by Western blotting using the monoclonal antibody TRAP3/10.| Product Name | PRR7 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [TRAP3/10] to PRR7PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.Entrez Gene (human): 5q35.3 Entrez Gene (mouse): 13 B1 |
| Immunogen | Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | TRAP3/10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB: Use at 1 µg/mL. Positive Control:murine brain lysate (red. Laemmli buffer): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | MGC:45012 TRAP TRAP3 |
|---|---|
| Target | PRR7 |
| Tissue Specificity | The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex. |
Application
Immunofluorescence staining of HEK-293 cells cotransfected with PRR7 / TRAP3 (red) and GFP-PSD-95 (green). PRR7 / TRAP3 detected by monoclonal antibody TRAP3/10. DNA visualized by DAPI (blue).
Application
Detection of PRR7 / TRAP3 in murine brain lysate by Western blotting using the monoclonal antibody TRAP3/10.| Application Notes | WB: Use at 1 µg/mL. Positive Control:murine brain lysate (red. Laemmli buffer): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
