Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.| Product Name | APRT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APRT, AMP, DKFZp686D13177, MGC125856, MGC125857, MGC129961, APRTD |
| Product description | Adenine phosphoribosyltransferase (APRT) belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.1) Silva, C.H., (2008) J. Biomol. Struct. Dyn. 25 (6), 589-597. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APRT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| APRT antibody can be used for detection of APRT by ELISA at 1:312500. APRT antibody can be used for detection of APRT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APRT |
|---|---|
| Alternative Names | APRT AMP DKFZp686D13177 MGC125856 MGC125857 MGC129961 APRTD |
| Molecular Weight(MW) | 20 kDa |
Application
Antibody used in WB on Human HEK293T cells at 0.2-1 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | APRT antibody can be used for detection of APRT by ELISA at 1:312500. APRT antibody can be used for detection of APRT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store APRT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
