Application
Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ASAH2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASAH2 Antibody: HNAC1, BCDase, LCDase, NCDase, N-CDase, HNAC1, Neutral ceramidase, Acylsphingosine deacylase 2 |
| Product description | ASAH2 Antibody: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.1) Nilsson A and Duan RD. Alkaline sphingomyelinases and ceramidases of the gastrointestinal tract. Chem. Phys. Lipids1999; 102:97-105. |
| Immunogen | ASAH2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human ASAH2. The immunogen is located within the last 50 amino acids of ASAH2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASAH2 |
|---|---|
| Alternative Names | ASAH2 Antibody: HNAC1 BCDase LCDase NCDase N-CDase HNAC1 Neutral ceramidase Acylsphingosine deacylase 2 |
Application
Western blot analysis of ASAH2 in 293 cell lysate with ASAH2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | ASAH2 antibody can be used for detection of ASAH2 by Western blot at 1 and 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ASAH2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ASAH2 Antibody is supplied in PBS containing 0.02% sodium azide. |
